Reduction of charge variants by CHO cell culture process optimization
نویسندگان
چکیده
منابع مشابه
P-66: Optimization of Human Luteinizing Hormone Expression in CHO Cells Culture by Stepwise Reduction in Serum Concentration
Background: Mammalian Cell lines are the main expression system for the production of recombinant therapeutic proteins. Optimization of cell culture condition is performed via alteration in different parameter. Cell culture media plays an important role in cell cycle because of compounds such as amino acids, vitamins, inorganic salts, glucose, and serum as a source of growth factors, trace elem...
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Bioprocess scale-up is a fundamental component of process development in the biotechnology industry. When scaling up a mammalian cell culture process, it is important to consider factors such as mixing time, oxygen transfer, and carbon dioxide removal. In this study, cell-free mixing studies were performed in production scale 5,000-L bioreactors to evaluate scale-up issues. Using the current bi...
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level: intermediAte Most biopharmaceutical production platforms are based on fed-batch cell culture protocols, which can support high volumetric productivity while maintaining low operational complexity (1). The industry is interested in developing or refining high-titer cell culture processes to meet increasing market demands and reduce manufacturing costs (2). Although advancements in cell en...
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ژورنال
عنوان ژورنال: Cytotechnology
سال: 2020
ISSN: 0920-9069,1573-0778
DOI: 10.1007/s10616-020-00375-x